eryC-5’ nuclease PCR: differentiating wildBrucella strains from vaccine strain S19
نویسندگان
چکیده
منابع مشابه
Genome Sequence of Brucella abortus Vaccine Strain S19 Compared to Virulent Strains Yields Candidate Virulence Genes
The Brucella abortus strain S19, a spontaneously attenuated strain, has been used as a vaccine strain in vaccination of cattle against brucellosis for six decades. Despite many studies, the physiological and molecular mechanisms causing the attenuation are not known. We have applied pyrosequencing technology together with conventional sequencing to rapidly and comprehensively determine the comp...
متن کاملDevelopment of a multiplex polymerase chain reaction assay for differentiation of field strain isolates and vaccine strains S19 and RB51 of Brucella in Iran
Bovine brucellosis is a zoonotic disease distributed worldwide and characterized by abortion and reducedfertility in cows. Since brucellosis eradication programme in Iran uses vaccination, test, slaughter andquarantine as control measures, it is essential to distinguish vaccine strains from strains that cause infectionsamong vaccinated cattle herds. We developed and evaluated a multiplex polyme...
متن کاملValidation of the abbreviated Brucella AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the vaccine strains, 19 and RB51.
The Brucella AMOS PCR assay was previously developed to identify and differentiate specific Brucella species. In this study, an abbreviated Brucella AMOS PCR test was evaluated to determine its accuracy in differentiating Brucella abortus into three categories: field strains, vaccine strain 19 (S19), and vaccine strain RB51/parent strain 2308 (S2308). Two hundred thirty-one isolates were identi...
متن کاملVector development for the expression of foreign proteins in the vaccine strain Brucella abortus S19.
A vector for the expression of foreign antigens in the vaccine strain Brucella abortus S19 was developed by using a DNA fragment containing the regulatory sequences and the signal peptide of the Brucella bcsp31 gene. This fragment was cloned in broad-host-range plasmid pBBR4MCS, resulting in plasmid pBEV. As a reporter protein, a repetitive antigen of Trypanosoma cruzi was used. The recombinant...
متن کامل5’-Nuclease PCR Assays for Foodborne Pathogen Detection using the Agilent-Stratagene Mx3000P Q-PCR System
Bacteriological culturing has traditionally been used to detect microbial pathogens in food, and this technique is still in widespread use today. However, the time-consuming and low-throughput nature of culture-based detection methods warrant their replacement by more rapid methods based on biomolecular analysis, like the polymerase chain reaction (PCR). PCR, predicated on DNA synthesis and amp...
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ژورنال
عنوان ژورنال: THE ASIAN JOURNAL OF ANIMAL SCIENCE
سال: 2014
ISSN: 0973-4791,0976-8963
DOI: 10.15740/has/tajas/9.2/207-211